Brodie D A
Tissue Cell. 1982;14(2):263-71. doi: 10.1016/0040-8166(82)90024-6.
Exposure of insect fat body to treatments which disrupt microtubules (colchicine, vinblastine sulfate and cold treatment) blocks intracellular transport between the Golgi complex and the plasma membrane but does not affect Golgi complex bead rings or transport from rough endoplasmic reticulum to the Golgi complex. Drugs which disrupt microfilaments (cytochalasins B and D) do not affect the bead rings or intracellular transport of secretory proteins at any level. Thus, intracellular transport between the rough endoplasmic reticulum and the Golgi complex and the arrangement of the beads in rings are both independent of the cytoskeleton. The ring arrangement is presumably maintained by interconnection(s) with rough endoplasmic reticulum membrane.
将昆虫脂肪体暴露于破坏微管的处理(秋水仙碱、硫酸长春碱和冷处理)中,会阻断高尔基体复合体与质膜之间的细胞内运输,但不影响高尔基体复合体的珠环或从粗面内质网到高尔基体复合体的运输。破坏微丝的药物(细胞松弛素B和D)在任何水平都不影响珠环或分泌蛋白的细胞内运输。因此,粗面内质网与高尔基体复合体之间的细胞内运输以及珠环的排列均与细胞骨架无关。珠环排列大概是通过与粗面内质网膜的连接来维持的。