秋水仙碱对大鼠肝细胞释放血浆蛋白的抑制作用。
Colchicine inhibition of plasma protein release from rat hepatocytes.
作者信息
Redman C M, Banerjee D, Howell K, Palade G E
出版信息
J Cell Biol. 1975 Jul;66(1):42-59. doi: 10.1083/jcb.66.1.42.
Abstract
Colchicine, both in vitro and in vivo, inhibits secretion of albumin and other plasma proteins. In vitro, secretion by rat liver slices is inhibited at 10-minus 6 M with maximal effect at 10-minus 5 M. Inhibition of secretion is accompanied by a concomitant retention of nonsecreted proteins within the slices. Colchicine does not inhibit protein synthesis at these concentrations. Vinblastine also inhibits plasma protein secretion but lumicolchicine, griseofulvin, and cytochalasin B do not. Colchicine also acts in vivo at 10-25 mumol/100 g body weight. Inhibition of secretion is not due to changes in the intracellular nucleotide phosphate levels. Colchicine, administered intravenously, acts within 2 min and its inhibitory effect lasts for at least 3 h. Colchicine has no effect on transport of secretory proteins in the rough or smooth endoplasmic reticulum but it causes these proteins to accumulate in Golgi-derived secretory vesicles.
摘要
秋水仙碱在体外和体内均能抑制白蛋白及其他血浆蛋白的分泌。在体外，大鼠肝切片的分泌在10⁻⁶ M浓度时受到抑制，在10⁻⁵ M浓度时效果最佳。分泌的抑制伴随着未分泌蛋白质在切片内的滞留。在这些浓度下，秋水仙碱不抑制蛋白质合成。长春花碱也抑制血浆蛋白分泌，但光秋水仙碱、灰黄霉素和细胞松弛素B则无此作用。秋水仙碱在体内以10 - 25 μmol/100 g体重的剂量起作用。分泌的抑制并非由于细胞内核苷酸磷酸水平的变化。静脉注射秋水仙碱后，2分钟内即可起效，其抑制作用至少持续3小时。秋水仙碱对粗面或滑面内质网中分泌蛋白的转运没有影响，但会使这些蛋白在源自高尔基体的分泌小泡中积累。
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