Peters J E, Steele W J
Biochem Pharmacol. 1982 Jun 1;31(11):2059-63. doi: 10.1016/0006-2952(82)90421-x.
Administration of ethanol thrice daily to rats in amounts sufficient to induce a high degree of physical dependence resulted in a 20% decrease in the rate of protein synthesis on liver membrane-bound polysomes in vivo after 3 days of treatment without affecting the rate on free polysomes. The inhibition was attributable to a decrease in the rate of polypeptide elongation as evidence by comparable decreases in nascent chain synthesis and completed protein release without any change in leucine uptake by liver. Chronic ethanol treatment did not affect the quantity or distribution of free and membrane-bound polysomes, the DNA concentration, or the weight of liver. The inhibition of protein synthesis on membrane-bound polysomes cannot, therefore, be readily ascribed to ethanol-induced nutritional deficiencies or to some nonspecific toxic effect of ethanol.
每天三次给大鼠投喂足以引起高度身体依赖性的乙醇，在处理3天后，体内肝膜结合多核糖体上的蛋白质合成速率降低了20%，而对游离多核糖体上的速率没有影响。这种抑制作用归因于多肽延伸速率的降低，新生链合成和完整蛋白质释放的可比降低证明了这一点，而肝脏对亮氨酸的摄取没有任何变化。慢性乙醇处理不影响游离和膜结合多核糖体的数量或分布、DNA浓度或肝脏重量。因此，膜结合多核糖体上蛋白质合成的抑制不能轻易归因于乙醇诱导的营养缺乏或乙醇的某些非特异性毒性作用。
