Fitchen J H, Cline M J
Exp Hematol. 1980 Jul;8(6):788-94.
We treated mice with high-dose busulfan to produce a residual or "latent" hematopoietic injury characterized by persistent normal or near-normal peripheral blood counts and marrow cellularity but moderate to marked reduction in hematopoietic stem cells. Control and "latent" mice were then challenged with glucan, a granulopoietic stimulant. We found that glucan administration induced a comparable increase in granulocytic differentiation in control and "latent" mice. This differentiational expansion was not accompanied by a further depletion of pluripotent hematopoietic stem cells. Our results fail to demonstrate competition between self-renewal and differentiation at the level of the CFUS, at least in this model. Stimuli for granulopoiesis act primarily on more distal, committed populations which are apparently unaffected by prior exposure to busulfan.
我们用高剂量白消安处理小鼠，以造成一种残留或“潜伏”的造血损伤，其特征为外周血计数和骨髓细胞数量持续正常或接近正常，但造血干细胞有中度至显著减少。然后用葡聚糖（一种粒细胞生成刺激剂）对对照小鼠和“潜伏”小鼠进行刺激。我们发现，给予葡聚糖后，对照小鼠和“潜伏”小鼠的粒细胞分化均有类似增加。这种分化性扩增并未伴随多能造血干细胞的进一步耗竭。至少在这个模型中，我们的结果未能证明在CFUS水平上自我更新与分化之间存在竞争。粒细胞生成刺激主要作用于更下游的定向群体，这些群体显然不受先前白消安暴露的影响。
