大鼠精子成熟过程中精子型己糖激酶活性的增加。

Increase in sperm type hexokinase activity of rat spermatozoa during maturation.

作者信息

Mongkolsirikieat S, Boonsaeng V

出版信息

Int J Androl. 1980 Dec;3(6):671-8. doi: 10.1111/j.1365-2605.1980.tb00154.x.

DOI:10.1111/j.1365-2605.1980.tb00154.x

PMID:7203684

Abstract

Rat hexokinases fro caput sperm (immature) and caudal sperm (mature) were investigated. The hexokinases from both sources were studied by DEAE-cellulose column chromatography and by cellulose acetate electrophoresis. The specific activity of caput sperm hexokinase was not significantly different from that of the caudal sperm enzyme. Spermatozoa possess two isozymes of hexokinase. Type I hexokinase was the predominant type in caput sperm whereas the sperm type of hexokinase was predominant in caudal sperm. Hexokinase types II and III were absent in both extracts.

摘要

对来自大鼠头部精子（未成熟）和尾部精子（成熟）的己糖激酶进行了研究。通过DEAE - 纤维素柱色谱法和醋酸纤维素电泳对这两种来源的己糖激酶进行了研究。头部精子己糖激酶的比活性与尾部精子酶的比活性没有显著差异。精子含有两种己糖激酶同工酶。I型己糖激酶是头部精子中的主要类型，而精子型己糖激酶在尾部精子中占主导地位。两种提取物中均不存在II型和III型己糖激酶。