Sundqvist T, Fjällbrant B, Magnusson K E
Int J Androl. 1981 Feb;4(1):18-24. doi: 10.1111/j.1365-2605.1981.tb00686.x.
The concentration of spermatozoa in human semen has been assessed by counting in the microscope with a hemocytometer and by counting with the Coulter Counter ZF. The Coulter Counter was supplied with a 100-channel pulseheight analyzer, Channelyzer C-1000, and a tape-punch for further handling of data in a desk-computer. It was found that the number of spermatozoa (less than 10(6) per ml) was over-estimated with the Coulter Counter. By subtraction of the non-spermatozoan background with the aid of mathematical curve-fitting procedures, these values could be corrected. To allow such an adequate analysis without the use of pulse-height analyzer and computer, the background corrections were simulated for the relative number of counts obtained at specified setting of the threshold levels (T) and other variables on the Coulter Counter ZF.
已通过使用血细胞计数器在显微镜下计数以及使用库尔特计数器ZF进行计数来评估人类精液中精子的浓度。库尔特计数器配备了100通道脉冲高度分析仪Channelyzer C - 1000和纸带穿孔机，以便在台式计算机中进一步处理数据。结果发现，库尔特计数器高估了精子数量（每毫升少于10⁶个）。借助数学曲线拟合程序减去非精子背景后，这些值可以得到校正。为了在不使用脉冲高度分析仪和计算机的情况下进行充分分析，针对在库尔特计数器ZF上特定阈值水平（T）设置和其他变量下获得的相对计数数量模拟了背景校正。
