Fairén A, Valverde F
J Comp Neurol. 1980 Dec 15;194(4):761-79. doi: 10.1002/cne.901940405.
The axonal arborization of chandelier cells is characterized by its conspicuous, vertically oriented, bouton aggregates. The efferent synaptic relationships established by these terminal formations were investigated by electron microscopy of Golgi preparations after gold toning and deimpregnation. In all cases examined from layers II and III of cat areas 17 and 18, the terminal formations, here denominated specific terminal portions (stp), make symmetric synapses upon axon initial segments of pyramidal neurons. Some identified stp's were reconstructed from ultrathin serial sections with the aid of a microcomputer-based system, and the number of synaptic contacts established on axon initial segments was evaluated. No evidence was found that parts of the axonal tree other than stp's also engage in synaptic contacts. Specific terminal portions are rather variable in complexity. However, the synaptic contacts they engage in are constant and the complexity of stp's from the same axonal arborization varies. It is, therefore, clear that all stp's are terminal axonal formations of a unique, specialized type of neuron. Computer techniques and conventional Golgi observations were used to study further details of chandelier cell morphology. Axonal plexuses are preferentially, although not exclusively, local and distribute within spheric, ovoid, or disk-shaped spaces. In most chandelier cells, the main axonal trunk descends to the white matter, where we have been unable to follow it further.
吊灯细胞的轴突分支以其明显的、垂直定向的终扣聚集体为特征。通过对经金染色和脱浸处理的高尔基染色标本进行电子显微镜观察，研究了这些终末结构所建立的传出突触关系。在对猫17区和18区第II和第III层检查的所有病例中，这里称为特定终末部分（stp）的终末结构，在锥体细胞的轴突起始段形成对称突触。借助基于微机的系统，从超薄连续切片中重建了一些已识别的stp，并评估了在轴突起始段建立的突触接触数量。未发现除stp之外的轴突树部分也参与突触接触的证据。特定终末部分在复杂性上有相当大的差异。然而，它们所参与的突触接触是恒定的，并且来自同一轴突分支的stp的复杂性也有所不同。因此，很明显所有的stp都是一种独特的、特化类型神经元的轴突终末结构。利用计算机技术和传统的高尔基染色观察方法进一步研究了吊灯细胞形态的细节。轴突丛优先（但非唯一）分布在局部，并分布在球形、卵形或盘形空间内。在大多数吊灯细胞中，主要轴突干向下延伸至白质，在那里我们无法进一步追踪它。
