Partlow L M, Bush L G, Stensaas L J, Kesner R P
J Histochem Cytochem. 1981 Jan;29(1):79-83. doi: 10.1177/29.1.7204948.
A novel quantitative method is described that makes it possible to routinely and accurately quantify contact autoradiographs of tissue sections in terms of actual amounts of radioactivity per square millimeter. Such determinations can either be made on the entire specimen or on any selected area of arbitrary shape that might correspond to a particular histological structure. This method utilizes the tissue specimens themselves as internal standards and requires the availability of a computerized image analyzer.
本文描述了一种新颖的定量方法，该方法能够依据每平方毫米的实际放射性量，对组织切片的接触放射自显影片进行常规且准确的定量分析。此类测定既可以针对整个标本进行，也可以针对任意形状的选定区域进行，这些区域可能对应特定的组织结构。该方法将组织标本自身用作内标，并且需要配备计算机化图像分析仪。
