Birkeland S A
J Immunol Methods. 1980;35(1-2):57-67. doi: 10.1016/0022-1759(80)90150-7.
Frozen stored lymphocytes are frequently used for sequential testing of immunological function in patients. The reliability of this system was investigated. By varying combinations of factors, it has been shown that lymphocyte samples, taken at different times and subsequently tested as a batch, give the most consistent results, the means of the various immune parameters measured on different sampling days in normal persons lying on straight lines parallel with the time axis. It is also shown that storage at -90 degrees C is equivalent to storage at -196.5% C.
冷冻保存的淋巴细胞常用于对患者免疫功能进行序贯检测。对该系统的可靠性进行了研究。通过改变多种因素的组合,结果表明,在不同时间采集并随后成批检测的淋巴细胞样本能给出最一致的结果,在正常人不同采样日测得的各种免疫参数的均值位于与时间轴平行的直线上。研究还表明,在-90℃保存等同于在-196.5℃保存。