一种用于检测天然细胞毒性的酶活性测定法。

An enzymatic assay for the detection of natural cytotoxicity.

作者信息

McCormick K J, Giovanella B C, Day D C, Parsons W R, Stehlin J S

出版信息

J Immunol Methods. 1980;35(1-2):83-90. doi: 10.1016/0022-1759(80)90152-0.

PMID:7204990

Abstract

The pronase-enhanced release of 14C-labeled DNA was used to assay cytotoxicity of murine natural killer cells. YAC-1 lymphoma cells were labeled with [14C]thymidine and incubated with increasing numbers of spleen cells from nude mice. At harvest, nuclease-free pronase was used to digest damaged target cells. Increases in cytotoxicity of 5--39% were obtained using optimal conditions. The reaction could be successfully inhibited with unlabeled YAC-1 cells. The degree of cytotoxicity was similar to that detected by 51Cr release, but a longer period of incubation was required.

摘要

用链霉蛋白酶增强释放的14C标记DNA来检测小鼠自然杀伤细胞的细胞毒性。YAC-1淋巴瘤细胞用[14C]胸腺嘧啶核苷标记，并与来自裸鼠的数量不断增加的脾细胞一起孵育。收获时，使用无核酸酶的链霉蛋白酶消化受损的靶细胞。在最佳条件下，细胞毒性增加了5%-39%。该反应可用未标记的YAC-1细胞成功抑制。细胞毒性程度与通过51Cr释放检测到的相似，但需要更长的孵育时间。