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一种用于同时分离人白细胞的简单技术。

A simple technique for simultaneous human leukocytes separation.

作者信息

Bicalho H M, Gontijo C M, Nogueira-Machado J A

出版信息

J Immunol Methods. 1981;40(1):115-6. doi: 10.1016/0022-1759(81)90087-9.

DOI:10.1016/0022-1759(81)90087-9
PMID:7204999
Abstract

By centrifuging heparinised human blood through a discontinuous Ficoll-Hypaque density gradient, it is possible to separate simultaneously mononuclear cells, neutrophils, and eosinophils.

摘要

通过在不连续的Ficoll-泛影葡胺密度梯度中对肝素化的人血进行离心，可以同时分离出单核细胞、中性粒细胞和嗜酸性粒细胞。

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1
A simple technique for simultaneous human leukocytes separation.一种用于同时分离人白细胞的简单技术。
J Immunol Methods. 1981;40(1):115-6. doi: 10.1016/0022-1759(81)90087-9.
2
Cellular activity of murine phagocytes isolated from peripheral blood by a discontinuous gradient.通过不连续梯度从外周血中分离的小鼠吞噬细胞的细胞活性。
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Superior leukocyte separation with a discontinuous one-step Ficoll-Hypaque gradient for the isolation of human neutrophils.采用不连续一步法Ficoll-泛影葡胺梯度分离法进行优质白细胞分离，用于分离人中性粒细胞。
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Separation of mononuclear and polymorphonuclear leucocytes from human blood by the one-step Hypaque-Ficoll method is dependent on blood column height.采用一步法聚蔗糖-泛影葡胺（Hypaque-Ficoll）从人血中分离单核细胞和多形核白细胞取决于血柱高度。
J Immunol Methods. 1982;48(1):81-5. doi: 10.1016/0022-1759(82)90212-5.
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Isolation of functional polymorphonuclear leukocytes from rhesus monkeys using discontinuous Ficoll-Hypaque density gradients.使用不连续的Ficoll-泛影葡胺密度梯度从恒河猴中分离功能性多形核白细胞。
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A single-step centrifugation method for separation of granulocytes and mononuclear cells from blood using discontinuous density gradient of Percoll.一种使用不连续Percoll密度梯度从血液中分离粒细胞和单核细胞的单步离心法。
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Mechanism of separation of polymorphonuclear leukocytes from whole blood by the one-step Hypaque-Ficoll method.采用一步法聚蔗糖-泛影葡胺法从全血中分离多形核白细胞的机制。
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Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method.使用聚蔗糖-泛影葡胺法从人血中同时纯化单核细胞和多形核白细胞的最佳条件。
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Preparative techniques influencing sedimentation of erythrocytes through the Hypaque-Ficoll medium used in the one-step technique for separation of human polymorphonuclear leukocytes from whole blood.在用于从全血中分离人多形核白细胞的一步法技术中，通过使用Hypaque-Ficoll介质影响红细胞沉降的制备技术。
J Immunol Methods. 1988 Jan 21;106(1):147-9. doi: 10.1016/0022-1759(88)90282-7.
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Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque.红细胞、粒细胞和单核白细胞在菲可-泛影葡胺不连续密度梯度上的一步分离。
J Immunol Methods. 1974 Aug;5(3):249-52. doi: 10.1016/0022-1759(74)90109-4.

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