Hoshino S, Nishimura M, Fukuyama K, Gellin G A, Epstein J H
J Invest Dermatol. 1981 Apr;76(4):231-8. doi: 10.1111/1523-1747.ep12526042.
Depigmentary effects of 4-tertiary butyl catechol (TBC) on UV-stimulated melanocytes on the flanks and naturally active melanocytes of ears were compared in Uscd strain hairless mice. UVB irradiation, twice a week for 1 or 2 mo, induced brown-black pigmentation on the flanks. A 1 M TBC application twice a week for 1 mo not only prevented the UV-stimulated pigmentation, but also promoted fading of the post-UV tanning. Dopa-stained split epidermal sheets showed a decrease in the number of melanocytes to less than one-half during the first month of TBC treatment. Melanocytes were often enlarged or lost their dendrites, and both premelanosomes and melanosomes showed ultrastructural changes. However, TBC application on the ears for 1 mo showed neither color change nor a decrease in the number of melanocytes. There were ultrastructural changes in melanocytes but the degree of abnormality was much less than those seen in UV-stimulated melanocytes. Continuation of TBC application for 2 mo with the UV irradiation on the flanks or the nonirradiated ears caused an increase in the number of melanocytes. These results suggest that the initial effect of TBC may be primarily cytotoxicity to melanocytes, and may correlate with their cellular functions. The stimulatory effects of TBC on melanocytes seen during the longer period of time requires further investigation.
在无毛Uscd品系小鼠中，比较了4-叔丁基邻苯二酚（TBC）对侧腹紫外线刺激的黑素细胞和耳部自然活跃黑素细胞的色素脱失作用。每周两次，持续1或2个月的UVB照射可诱导侧腹出现棕黑色色素沉着。每周两次涂抹1 M TBC，持续1个月，不仅可预防紫外线刺激的色素沉着，还可促进紫外线后晒黑的消退。多巴染色的分离表皮片显示，在TBC治疗的第一个月内，黑素细胞数量减少至不到一半。黑素细胞常肿大或失去树突，前黑素小体和黑素小体均显示超微结构变化。然而，在耳部涂抹TBC 1个月，既未出现颜色变化，黑素细胞数量也未减少。黑素细胞有超微结构变化，但异常程度远低于紫外线刺激的黑素细胞。在侧腹进行紫外线照射或未照射的耳部继续涂抹TBC 2个月，会导致黑素细胞数量增加。这些结果表明，TBC的初始作用可能主要是对黑素细胞的细胞毒性，且可能与其细胞功能相关。TBC在较长时间内对黑素细胞的刺激作用需要进一步研究。
