Schlebusch H, Sorger M, Munz E, Kessler A C, Zwez W
J Clin Chem Clin Biochem. 1980 Dec;18(12):885-91.
The determination of glucose in hemolysed and stabilized blood samples by the hexokinase/glucose-6-phosphate-dehydrogenase-method is described. The glucose concentration in hemolysed blood samples was stable for 7 days. Even at high glucose concentrations (linear range up to 60 mmol/l), the reaction came to completion within 5 minutes. No interference by lipemia, bilirubin and drugs was observed; the interference of fructose was slight. Compared with deproteinized samples there was a very good correlation between this method and the reference method. Precision and recovery were good. This method is also suited for the analysis of few samples and offers the possibility of blood sugar self-profiles in diabetic out-patients and gives an increasing improvement of diabetic control.
描述了用己糖激酶/葡萄糖-6-磷酸脱氢酶法测定溶血且稳定的血样中的葡萄糖。溶血血样中的葡萄糖浓度在7天内保持稳定。即使在高葡萄糖浓度下(线性范围高达60 mmol/l),反应也能在5分钟内完成。未观察到脂血、胆红素和药物的干扰;果糖的干扰很轻微。与去蛋白样品相比,该方法与参考方法之间具有很好的相关性。精密度和回收率良好。该方法也适用于少量样品的分析,并为糖尿病门诊患者提供了血糖自我监测的可能性,且能不断改善糖尿病的控制情况。