Katsuta H, Takaoka T, Huh N
Jpn J Exp Med. 1980 Oct;50(5):329-37.
Three cells strains of tissue culture were established from normal fetal human liver and kidney tissues. The rate of cell proliferation decreased with time after the primary culture. The cells were rarely subcultured, but the medium was renewed routinely twice a week. After 9 months of cultivation, the cells were found in both tissues to have abruptly begun to proliferate rapidly, but only in the group culture in the medium containing galactose and sodium pyruvate in place of glucose. No special treatment were given to these cultures, e.g. treatment with viruses, chemical carcinogens and others. The chromosome number was kept around diploid in the beginning but was shifted to hypotriploid after the establishment. The cell strain from liver consists of epithelial cells. The cell strains from kidney consist of mixed population of various kinds of cells. Doubling time of cells is about 33.4 hours in all of them, as determined by cinemicrography. This might be the first establishment of cell strains from untreated, normal human tissues.
从正常胎儿的肝脏和肾脏组织中建立了三株组织培养细胞系。原代培养后细胞增殖速率随时间下降。细胞很少传代，但培养基每周常规更换两次。培养9个月后，发现两种组织中的细胞突然开始快速增殖，但仅在含有半乳糖和丙酮酸钠而非葡萄糖的培养基中进行群体培养时出现这种情况。对这些培养物未进行特殊处理，例如用病毒、化学致癌物等处理。开始时染色体数保持在二倍体左右，但建立后转变为亚三倍体。肝脏来源的细胞系由上皮细胞组成。肾脏来源的细胞系由各种细胞的混合群体组成。通过显微电影摄影术测定，所有细胞系的细胞倍增时间约为33.4小时。这可能是首次从未经处理的正常人体组织中建立细胞系。
