Salas-Prato M, Tanguay J F, Lefebvre Y, Wojciechowicz D, Liem H H, Barnes D W, Ouellette G, Muller-Eberhard U
Research Center, Notre-Dame Hospital, Montreal, Quebec, Canada.
In Vitro Cell Dev Biol. 1988 Mar;24(3):230-8. doi: 10.1007/BF02623552.
We established for human fetal liver cells (cultured for 2 wk) in a hormonally defined medium, optimal conditions for attachment, multiplication, and preservation of epithelial morphology as well as production and secretion of serum proteins characteristic of fetal (alpha l-fetoprotein, AFP) and adult (albumin and hemopexin) life. Conditions were considered optimal when cell number, albumin, and hemopexin levels were maintained throughout the 2-wk culture period. However, the decrease in AFP concentration, which occurred after a few days of culture, could not be reversed. The culture system developed is a suitable model for studying regulatory mechanisms governing structure and function during differentiation and may prove useful for testing the effect of toxic agents during fetal development of the human liver.
我们在一种激素限定培养基中为人类胎儿肝细胞（培养2周）建立了附着、增殖和维持上皮形态的最佳条件，以及胎儿期（甲胎蛋白，AFP）和成人生理期（白蛋白和血红素结合蛋白）特征性血清蛋白的产生和分泌条件。当在整个2周培养期内细胞数量、白蛋白和血红素结合蛋白水平保持稳定时，这些条件被认为是最佳的。然而，培养几天后出现的AFP浓度下降无法逆转。所开发的培养系统是研究分化过程中结构和功能调控机制的合适模型，并且可能被证明对测试人类肝脏胎儿发育期间有毒物质的影响有用。
