多聚核糖核苷酸中存在1,N6-乙烯腺苷或3,N4-乙烯胞苷导致的转录错误和模糊性。
Transcriptional errors and ambiguity resulting from the presence of 1,N6-ethenoadenosine or 3,N4-ethenocytidine in polyribonucleotides.
作者信息
Spengler S, Singer B
出版信息
Nucleic Acids Res. 1981 Jan 24;9(2):365-73. doi: 10.1093/nar/9.2.365.
Abstract
1,N6-Ethenoadenosine (epsilon A) and 3,N4-Ethenocytidine (epsilon C) in copolymers with unmodified nucleosides were transcribed using DNA-dependent RNA polymerase in the presence of Mn2+. Nearest neighbor analysis of the products showed that epsilon A directed incorporation of A much greater than U greater than C while epsilon C directed the incorporation of U greater than or equal to A much greater than C Neither directed G into the complementary polymer. Such misincorporations resulting from epsilon A and epsilon C, compounds that are formed in vivo by the carcinogen vinyl chloride, may have a biological role as promutagens.
摘要
在存在锰离子的情况下，使用依赖DNA的RNA聚合酶转录了与未修饰核苷形成共聚物的N6-乙烯基腺苷（εA）和3,N4-乙烯基胞苷（εC）。对产物的邻位分析表明，εA指导A的掺入量远大于U大于C，而εC指导U的掺入量大于或等于A远大于C，两者均未将G掺入互补聚合物中。由εA和εC导致的这种错误掺入，这两种化合物是由致癌物氯乙烯在体内形成的，可能作为促诱变剂具有生物学作用。
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