Goldenberg D M, Pavia R A
Science. 1981 Apr 3;212(4490):65-7. doi: 10.1126/science.7209521.
Human malignant cancer tumors grafted into nude mice produce tumors containing both human cancer cells and the host's stromal cells. After short-term propagation of these tumors in vitro, the murine mesenchymal cells appear transformed and are tumorigenic in nude mice. However, established human cancer cell lines fail to similarly after adjacent murine stromal cells when used to produce tumors in nude mice. These experiments suggest that cancer cells may recruit normal cells to become malignant, qualifying the view of the clonal (unicellular) origin of cancer.
移植到裸鼠体内的人类恶性肿瘤会产生包含人类癌细胞和宿主基质细胞的肿瘤。这些肿瘤在体外短期传代后，鼠间充质细胞似乎发生了转化，并且在裸鼠体内具有致瘤性。然而，已建立的人类癌细胞系在用于在裸鼠体内产生肿瘤时，不会以类似方式招募相邻的鼠基质细胞。这些实验表明癌细胞可能招募正常细胞使其恶变，这对癌症的克隆（单细胞）起源观点提出了质疑。
