Herve P, Lamy B, Peters A, Toubin M, Bidet A C
Vox Sang. 1980 Oct;39(4):195-204. doi: 10.1111/j.1423-0410.1980.tb01857.x.
Red blood cells (RBC) were collected with citrate-phosphate-dextrose (CPD) in a blood-pack optimal additive system. After concentration to 90% hematocrit they were diluted with saline-adenine-glucose medium (SAG-RBC), and stored for 35 days. In this work the RBC were stored in the presence of leukocytes. The SAG medium allows RBC conservation during 35 days at +4 degrees C. The adenosine triphosphate (ATP) level of RBC is compatible with their survival. During the first 2 weeks, hemolysis of SAG-RBC was not greater than in CPD blood. Nevertheless, hemolysis reached 1.49% on day 35, and there was a marked increase in RBC osmotic fragility. Scanning electron-microscopic studies of 35-day RBC showed that the majority of them became echinocytes. After incubation in fresh frozen plasma, the RBC recovered satisfactory osmotic resistance and normal disc shape. The post-transfusion viability was normal with greater than 70% recovery after 48 h. The in vivo restoration of 2,3-diphosphoglycerate (2,3-DPG) was rapid in the transfused SAG-RBC, 50% of the initial 2,3-DPG level being restored in 1 h. The in vivo studies proved that the functional quality of these RBC was compatible with their use in transfusion. The most important problem concerns the supernatant hemoglobin level of the SAG-RBC to be used for massive transfusion.
红细胞（RBC）采用枸橼酸盐-磷酸盐-葡萄糖（CPD）在血袋最佳添加剂系统中采集。浓缩至血细胞比容为90%后，用生理盐水-腺嘌呤-葡萄糖培养基（SAG-RBC）稀释，并储存35天。在本研究中，红细胞在白细胞存在的情况下储存。SAG培养基可使红细胞在4℃下保存35天。红细胞的三磷酸腺苷（ATP）水平与其存活情况相符。在最初的2周内，SAG-RBC的溶血率不高于CPD血液。然而，在第35天溶血率达到1.49%，且红细胞渗透脆性显著增加。对储存35天的红细胞进行扫描电子显微镜研究表明，大多数红细胞变成了棘状细胞。在新鲜冷冻血浆中孵育后，红细胞恢复了令人满意的渗透压耐受性和正常的圆盘状形态。输血后存活率正常，48小时后恢复率超过70%。在输注的SAG-RBC中，2,3-二磷酸甘油酸（2,3-DPG）的体内恢复迅速，1小时内可恢复至初始2,3-DPG水平的50%。体内研究证明，这些红细胞的功能质量与其用于输血的用途相符。最重要的问题涉及用于大量输血的SAG-RBC的上清血红蛋白水平。
