Gulliksson H, Karlman G, Segerlind A, Gullbring B
Vox Sang. 1986;50(1):16-20. doi: 10.1111/j.1423-0410.1986.tb04839.x.
The content of microaggregates was determined in leukocyte-poor red blood cells (RBC) after 5 weeks of storage in citrate-phosphate-dextrose (CPD)-adenine plasma and in saline-adenine-glucose-mannitol (SAGM) medium. The size range was 3,600-200,000 fl, corresponding to a particle diameter of 19-73 micron, presuming a spherical particle shape. In comparison with CPD-adenine whole blood (100%), the total number of particles per unit of RBC was found to be 41.2% (CPD-adenine anticoagulant) and 25.4% (SAGM medium). The corresponding data for total particle volumes were 43.3 and 30.3%. The significant differences between the two categories of RBC could not be explained on the basis of differences concerning pH or cell composition. A majority of particles (50-62%) was observed in the lowest size range (19-29 microns), corresponding to only 18-26% of the total particle volume. The accumulation of di-2-ethylhexylphthalate (DEHP) was determined in two variations of RBC stored in SAGM medium and in RBC stored in CPD-adenine plasma. The quantities of DEHP per unit of RBC after 5 weeks were significantly smaller in SAGM medium (9.0 +/- 3.6 and 22.7 +/- 6.1 mg, respectively) compared to CPD-adenine plasma (30.1 +/- 9.5 mg).
在柠檬酸盐 - 磷酸盐 - 葡萄糖（CPD） - 腺嘌呤血浆和生理盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇（SAGM）培养基中储存5周后，测定了贫白细胞红细胞（RBC）中微聚集体的含量。尺寸范围为3600 - 200,000飞升，假设颗粒为球形，则对应于19 - 73微米的粒径。与CPD - 腺嘌呤全血（100%）相比，每单位RBC的颗粒总数分别为41.2%（CPD - 腺嘌呤抗凝剂）和25.4%（SAGM培养基）。总颗粒体积的相应数据分别为43.3%和30.3%。两类RBC之间的显著差异无法根据pH值或细胞组成的差异来解释。在最低尺寸范围（19 - 29微米）观察到大多数颗粒（50 - 62%），其仅占总颗粒体积的18 - 26%。测定了储存在SAGM培养基中的两种RBC变体以及储存在CPD - 腺嘌呤血浆中的RBC中邻苯二甲酸二（2 - 乙基己基）酯（DEHP）的累积量。5周后，与CPD - 腺嘌呤血浆（30.1±9.5毫克）相比，SAGM培养基中每单位RBC的DEHP量显著较小（分别为9.0±3.6和22.7±6.1毫克）。
