Desgeorges P T, Ambroise-Thomas P, Falanga P, Renversez J C
Ann Biol Clin (Paris). 1980;38(6):361-3.
The authors describe a modified Gedelisa test made up an electrophorese in gradient polyacrylamide gel plus an Elisa test. The gel gradient, after electrophoretic migration is cut longitudinaly. One half is stained with Coomassie blue and the second half is cut in slices of 1 mm thick. Each slice is placed in a well of a microtitration polystirene plate, filled up with carbonate buffer pH 9,6 and then scrached. Proteins diffuse out of the gel and coated on the polystirene. The antigenicity of the coated proteins is revealed by Elisa test. This technique was applied to the study of Toxoplasma gondii exo-antigens obtained from medium of in vitro culture on Vero cells. This antigen contains four fractions, three of them are stained with Coomassie blue, their molecular weights are 160 000, 830 000 and more than one million daltons.
作者描述了一种改良的格德利萨试验，该试验由梯度聚丙烯酰胺凝胶电泳和酶联免疫吸附测定（ELISA）试验组成。电泳迁移后的凝胶梯度纵向切割。一半用考马斯亮蓝染色，另一半切成1毫米厚的薄片。将每片薄片置于微量滴定聚苯乙烯板的孔中，用pH 9.6的碳酸盐缓冲液填充，然后刮擦。蛋白质从凝胶中扩散出来并包被在聚苯乙烯上。通过ELISA试验揭示包被蛋白质的抗原性。该技术应用于对从Vero细胞体外培养基中获得的弓形虫外抗原的研究。该抗原包含四个组分，其中三个组分用考马斯亮蓝染色，它们的分子量分别为160000、830000和超过一百万个道尔顿。
