Eugster A K
Am J Vet Res. 1980 Dec;41(12):2020-4.
parvoviruses isolated from the intestines of dogs that died of an enteric infection were propagated in various parasynchronized canine and feline cell cultures. Viral antigen could be visualized in infected cell cultures with the aid of fluorescein-labeled feline, as well as porcine, parvovirus antisera, and in an indirect test with sera from dogs that had recovered from a parvovirus infection. The virus hemagglutinated porcine RBC at 4 C and 25 C but not at 37 C. An inactivated canine parvovirus vaccine elicited an immune response, but no adverse reactions, when inoculated into dogs. Vaccinated dogs were immune and did not show any clinical signs when challenge exposed with virulent virus, whereas nonvaccinated, nonimmune dogs became clinically ill when inoculated with the same virus. Humoral hemagglutination-inhibiting parvovirus antibody values corresponded well with susceptibility and resistance to experimental inoculation of dogs with canine parvoviruses.
从死于肠道感染的犬类肠道中分离出的细小病毒，在各种同步化的犬类和猫类细胞培养物中进行繁殖。借助荧光素标记的猫细小病毒抗血清以及猪细小病毒抗血清，在感染的细胞培养物中可观察到病毒抗原，并且在对已从细小病毒感染中康复的犬类血清进行的间接检测中也能观察到。该病毒在4℃和25℃时能凝集猪红细胞，但在37℃时不能。一种灭活的犬细小病毒疫苗接种到犬类体内时可引发免疫反应，但无不良反应。接种疫苗的犬类具有免疫力，在用强毒病毒进行攻击暴露时未表现出任何临床症状，而未接种疫苗、无免疫力的犬类在接种相同病毒时则出现临床病症。体液中血凝抑制细小病毒抗体值与犬对犬细小病毒实验接种的易感性和抗性密切相关。
