Diekert G, Gilles H H, Jaenchen R, Thauer R K
Arch Microbiol. 1980 Dec;128(2):256-62. doi: 10.1007/BF00406169.
Factors F430 from methanogenic bacteria have recently been shown to contain nickel and it has been speculated that they may have a nickel tetrapyrrole structure. This assumption was tested by determining whether succinate is incorporated by growing Methanobacterium thermoautotrophicum into three factors F430. Succinate is assimilated by Methanobacterium thermoautotrophicum into the amino acids glutamate, arginine and proline and into tetrapyrroles rather than other cell components. It was found that per mol nickel 8--9 mol of succinate were incorporated into the three factors F430 which is the amount predicted for a tetrapyrrole structure. Since the three factors F430 only contained significant amounts of glutamate rather than arginine or proline, the incorporation data suggest that factors F430 are nickel tetrapyrrole compounds. Spectral properties of the three factors F430, apparent molecular weights, and the absence of phosphor in these compounds are also described.
最近研究表明，产甲烷菌中的F430因子含有镍，据推测它们可能具有镍四吡咯结构。通过测定自养嗜热甲烷杆菌在生长过程中是否将琥珀酸掺入三种F430因子中来验证这一假设。自养嗜热甲烷杆菌将琥珀酸同化为氨基酸谷氨酸、精氨酸和脯氨酸以及四吡咯，而非其他细胞成分。研究发现，每摩尔镍有8 - 9摩尔琥珀酸掺入三种F430因子中，这是四吡咯结构所预测的量。由于三种F430因子仅含有大量的谷氨酸，而非精氨酸或脯氨酸，掺入数据表明F430因子是镍四吡咯化合物。文中还描述了三种F430因子的光谱特性、表观分子量以及这些化合物中不含磷的情况。
