Diekert G, Konheiser U, Piechulla K, Thauer R K
J Bacteriol. 1981 Nov;148(2):459-64. doi: 10.1128/jb.148.2.459-464.1981.
Methanobacterium thermoautotrophicum has been reported to require nickel for growth and to contain high concentrations of a nickel tetrapyrrole designated factor F430. In this communication it is shown that all methanogenic bacteria investigated incorporated nickel during growth and also synthesized factor F430. This was also true for Methanobrevibacter smithii, which is dependent on acetate as a carbon source, and for Methanosarcina barkeri growing on acetate or methanol as energy sources. Other bacteria, including Acetobacterium woodii and Clostridium thermoaceticum, contained no factor F430. It is further shown that two yellow nickel-containing degradation products were formed from factor F430 when heated at pH 7. This finding explains why several forms of factor F430 were found in methanogenic bacteria when a heat step was employed in the purification procedure.
据报道，嗜热自养甲烷杆菌生长需要镍，并含有高浓度的一种名为因子F430的镍四吡咯。在本通讯中表明，所有研究的产甲烷细菌在生长过程中都摄取镍，并且还合成因子F430。依赖乙酸盐作为碳源的史氏甲烷短杆菌以及以乙酸盐或甲醇作为能源生长的巴氏甲烷八叠球菌也是如此。其他细菌，包括伍氏乙酸杆菌和热乙酸梭菌，不含因子F430。进一步表明，因子F430在pH 7加热时会形成两种含镍的黄色降解产物。这一发现解释了为什么在纯化过程中采用加热步骤时，在产甲烷细菌中发现了几种形式的因子F430。
