冷冻对通过放射免疫测定法测定的尿中Tamm-Horsfall糖蛋白估计量的影响。
Effects of freezing on the estimated amounts of Tamm--Horsfall glycoprotein in urine, as determined by radioimmunoassay.
作者信息
Goodall A A, Marshall R D
出版信息
Biochem J. 1980 Sep 1;189(3):533-9. doi: 10.1042/bj1890533.
Abstract
Freeze-drying or freezing of salt-free solutions of human Tamm--Horsfall glycoprotein appeared to lead to changes in the structure of the latter, changes that increased its ability to bind with antibody raised, in rabbits, against it. This alteration in avidity of the glycoprotein was observed irrespective of whether antiserum was raised against freeze-dried or non-frozen antigen. The implications of this finding for the radioimmunoassay of the glycoprotein in urine samples were studied. Appropriate treatment for urine samples, before assay, was devised. The amount of Tamm--Horsfall glycoprotein excreted was shown to range from 30 to 138 mg in normal males and 43 to 126 mg in normal females per 24 h.
摘要
人Tamm-Horsfall糖蛋白无盐溶液的冻干或冷冻似乎会导致该糖蛋白结构发生变化，这些变化增强了它与在兔体内产生的针对它的抗体结合的能力。无论抗血清是针对冻干抗原还是非冷冻抗原产生的，均观察到该糖蛋白亲和力的这种改变。研究了这一发现对尿液样本中该糖蛋白放射免疫测定的意义。设计了在测定前对尿液样本进行的适当处理方法。结果表明，正常男性每24小时排出的Tamm-Horsfall糖蛋白量为30至138毫克，正常女性为43至126毫克。
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