Cullis C A
Biochim Biophys Acta. 1981 Jan 29;652(1):1-15. doi: 10.1016/0005-2787(81)90203-3.
The complexity of the flax genome has been determined by reassociation kinetics. The total complexity of one constituent genome was 3.5 . 10(8) nucleotide pairs. The single copy sequences comprised 44% of the genome and showed a long period interspersion pattern with the repetitive sequences. The repetitive sequences occurred in clusters which stretched for at least 10 000 base pairs. Within these clusters the individual repetitive elements were about 650 base pairs. These elements themselves showed little interspersion of different frequency classes in lengths less than 3000 base pairs. The repetitive sequence duplexes formed on reassociation, except for the satellite DNA, showed a high thermal stability. The fold-back DNA comprised 1% of the total genome, and was itself clustered in a small fraction of the genome.
亚麻基因组的复杂性已通过重缔合动力学确定。一个组成基因组的总复杂性为3.5×10⁸个核苷酸对。单拷贝序列占基因组的44%，并与重复序列呈现长周期散布模式。重复序列成簇出现，这些簇至少延伸10000个碱基对。在这些簇内，单个重复元件约为650个碱基对。这些元件本身在长度小于3000个碱基对时，不同频率类别的散布很少。重缔合时形成的重复序列双链体，除卫星DNA外，表现出高热稳定性。回文DNA占基因组总量的1%，且自身聚集在基因组的一小部分中。
