Hülsmann W C, Breeman W A, Stam H, Kort W J
Biochim Biophys Acta. 1981 Feb 23;663(2):373-9. doi: 10.1016/0005-2760(81)90166-1.
Chylomicrons were isolated from the urine of rats after a surgical procedure in which the cysterna chyli was connected with the right ureter. The fatty acids of the chylomicrons served as a respiratory substrate for rat heart and not for rat small intestine during in vitro vascular perfusions. The reason for the absence of chylomicron utilization in small intestine was found to be the virtual absence of lipoprotein lipase from this organ. Both heart and small intestine oxidized oleate complexed to albumin. Increasing the molar ratio of fatty acid to albumin from 3 to 6 did not affect the rate of fatty acid oxidation in heart, but increased fatty acid oxidation in small intestine.
通过一种手术程序，即连接乳糜池与右输尿管后，从大鼠尿液中分离出乳糜微粒。在体外血管灌注过程中，乳糜微粒的脂肪酸可作为大鼠心脏而非大鼠小肠的呼吸底物。发现小肠中未利用乳糜微粒的原因是该器官几乎不存在脂蛋白脂肪酶。心脏和小肠都氧化与白蛋白结合的油酸。将脂肪酸与白蛋白的摩尔比从3增加到6并不影响心脏中脂肪酸的氧化速率，但增加了小肠中脂肪酸的氧化。
