Dunbar J C, Ralston G B
Biochim Biophys Acta. 1981 Jan 30;667(1):177-84. doi: 10.1016/0005-2795(81)90078-7.
The hydrodynamic properties of the spectrin dimer have been examined. The S20,w value of 9.3 S and the D20,w value of 1.75 x 10(-7) cm2 x s-1 yield a molecular weight of 470 000, in good agreement with the value from sedimentation equilibrium of 460 000. The frictional ratio of 2.3 and the intrinsic viscosity of 36 ml/g are not consistent with a compact, globular structure, but the value of the parameter Ks/[eta], 1.15, is not consistent with a rigid rod model. The most appropriate model, consistent also with data from other laboratories, is of a flexible, kinked rod-like molecule.
已经对血影蛋白二聚体的流体动力学性质进行了研究。9.3 S的S20,w值和1.75×10(-7) cm2×s-1的D20,w值得出分子量为470 000，与沉降平衡法得出的460 000的值非常吻合。2.3的摩擦比和36 ml/g的特性粘度与紧密的球状结构不一致，但参数Ks/[η]的值1.15与刚性杆模型不一致。与其他实验室的数据也一致的最合适模型是一个柔性的、有扭结的杆状分子。
