[大鼠脑碱性核糖核酸酶的动力学特性]
[Kinetic properties of alkaline ribonuclease from rat brain].
作者信息
Shelepina E P
出版信息
Biokhimiia. 1980 Jan;45(1):137-41.
PMID:7213829
Abstract
The rate of hydrolysis of polymeric yeast RNA by highly purified preparation of alkaline RNAse from the post-mitochondrial cellular fraction from the large hemispheres of rat brain was studied. It was shown that the reaction rate is maximal at the substrate concentration of 1-2 mg/ml. The curve of the reaction rate versus substrate concentration was found to be S-shaped. The value of S0,5 was equal to 0,138 mg/ml. The inhibition of alkaline RNAse by its specific protein inhibitor was of a non-competitive type.
摘要
研究了用大鼠大脑大半球线粒体后细胞组分高度纯化的碱性核糖核酸酶制剂对聚合酵母核糖核酸的水解速率。结果表明，在底物浓度为1-2mg/ml时反应速率最大。发现反应速率与底物浓度的曲线呈S形。S0.5的值等于0.138mg/ml。碱性核糖核酸酶被其特异性蛋白质抑制剂抑制属于非竞争性类型。
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