[费氏弧菌发光电子传递链的抑制分析]
[Inhibitory analysis of the luminescent electron transport chain of Photobacterium fischeri].
作者信息
Ismailov A D, Danilov V S, Malkov Iu A, Egorov N S
出版信息
Biokhimiia. 1981 Jan;46(1):40-6.
PMID:7248374
Abstract
The quenching of luminescence of bacterial luciferase from Photobacterium fischeri by non-specific electron acceptors and inhibitors of dehydrogenases was studied. The inhibition of the luminescent reaction obeys the non-competitive mechanism with NADH, FMN and aliphatic aldehyde. The inhibitors compete with cytochrome c for NADH -- cytochrome c oxido-reductase. It is concluded that lumiredoxin, a FeS-containing protein, is the most sensitive component of the luminescent electron transport chain.
摘要
研究了非特异性电子受体和脱氢酶抑制剂对费氏弧菌细菌荧光素酶发光的猝灭作用。发光反应的抑制遵循与NADH、FMN和脂肪醛的非竞争性机制。抑制剂与细胞色素c竞争NADH-细胞色素c氧化还原酶。得出的结论是，含FeS的蛋白质——发光铁氧还蛋白是发光电子传递链中最敏感的成分。
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