Ben-Ishay Z, Prindull G, Ben-Israel D
Blut. 1981 Mar;42(3):165-72. doi: 10.1007/BF01026386.
Pluripotent hematopoietic stem cells from the bone marrow of newborn mice (9--13 days of age) have been studied following the administration of hydroxyurea (HU, 1,000 mg/kg). Following a single injection as well as a regimen of eight injections complete bone marrow reconstitution was achieved after 7 days. During the recovery phase diffusion chamber progenitor cells (DCPC) from pretreated newborn mouse bone marrow proliferated strongly to give significantly higher cell yields than DCPC from control animals. By contrast (colony-forming units-spleen [CFU-S]) levels were reduced at the same time to between 15 and 40% of controls suggesting a small-sized reserve pool of resting CFU-S in newborn mice. Assuming that DCPC are less differentiated than CFU-S and are non-cycling stem cells they may possibly pass through the CFU-S stage rapidly in newborn mice under the stress of hematopoietic reconstitution and proceed directly to myelopoiesis.
对新生小鼠（9 - 13日龄）骨髓中的多能造血干细胞在给予羟基脲（HU，1000mg/kg）后进行了研究。单次注射以及八次注射方案后，7天后实现了完全的骨髓重建。在恢复阶段，预处理新生小鼠骨髓中的扩散盒祖细胞（DCPC）强烈增殖，产生的细胞产量显著高于对照动物的DCPC。相比之下，同期脾集落形成单位（CFU - S）水平降至对照的15%至40%，这表明新生小鼠中存在一个小型的静止CFU - S储备池。假设DCPC比CFU - S分化程度低且是不循环的干细胞，那么在造血重建的应激下，它们可能在新生小鼠中迅速通过CFU - S阶段，直接进入髓系造血。
