Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A
Can J Microbiol. 1980 Nov;26(11):1259-63. doi: 10.1139/m80-210.
Two main physiological groups of bacteria are known which are adapted to high saline environments, moderate and extreme halophiles. In order to clarify some aspects of the competition between these two groups in their natural habitats, continuous cultures were used to provide a changing spectrum of conditions of salt concentration, temperature, and nutrient concentration (dilution rate). The effects of these parameters on natural solar saltern populations were studied. Complex media were used to increase the range of competing microorganisms. Nineteen strains of halophilic bacteria were isolated and studied with respect to their growth response at different salt concentrations. The temperature seemed to be the decisive factor within the range of salt concentrations studied (20-30%, w/v), the moderate halophiles being favored by low temperatures. Within this group, motile, gram-negative rods, and spiral forms were the predominant morphological types. In general, microorganisms that showed high growth rates in batch cultures predominated in continuous cultures with high dilution rates (high nutrient concentrations); those that grew slowly in batch cultures predominated in cultures with low dilution rates.
已知有两大类生理菌群适应高盐环境，即中度嗜盐菌和极端嗜盐菌。为了阐明这两类菌群在其自然栖息地的竞争情况，采用连续培养法来提供盐浓度、温度和营养浓度（稀释率）不断变化的条件范围。研究了这些参数对天然太阳盐场菌群的影响。使用复杂培养基以增加竞争微生物的种类。分离出19株嗜盐菌，并研究了它们在不同盐浓度下的生长反应。在所研究的盐浓度范围内（20 - 30%，w/v），温度似乎是决定性因素，低温有利于中度嗜盐菌生长。在这一类群中，运动性革兰氏阴性杆菌和螺旋形态是主要的形态类型。一般来说，在分批培养中生长速率高的微生物在高稀释率（高营养浓度）的连续培养中占主导；在分批培养中生长缓慢的微生物在低稀释率培养中占主导。
