Raju B C, Nyland G, Meikle T, Purcell A H
Can J Microbiol. 1981 Feb;27(2):249-53. doi: 10.1139/m81-038.
Spiroplasmas were cultured from nonsurface-sterilized flowers of magnolia tree (Magnolia grandiflora L.) and tulip tree (Liriodendron tulipfera L.) in Alameda, Solano, and Yolo counties in California. Spiroplasmas were also isolated from honey bees (Apis mellifera L.) collected in the vicinity of Davis and Berkeley, CA. Most of the isolates grew relatively slowly at 31 or 37 degrees C reaching maximum population at 31 degrees C in 8-10 days. The flower isolates were serologically indistinguishable by deformation and growth inhibition tests of spiroplasma strain AS-576 from honey bee from Maryland. Isolates from honey bees were serologically closely related to spiroplasma strain SE-3 from tulip tree flower from Connecticut.
从加利福尼亚州阿拉米达县、索拉诺县和约洛县的木兰树（Magnolia grandiflora L.）和鹅掌楸树（Liriodendron tulipfera L.）未进行表面消毒的花朵中培养出了螺原体。还从加利福尼亚州戴维斯和伯克利附近采集的蜜蜂（Apis mellifera L.）中分离出了螺原体。大多数分离株在31或37摄氏度下生长相对缓慢，在31摄氏度下8 - 10天达到最大种群数量。通过对来自马里兰州蜜蜂的螺原体菌株AS - 576进行变形和生长抑制试验，发现从花朵中分离出的菌株在血清学上无法区分。从蜜蜂中分离出的菌株在血清学上与来自康涅狄格州鹅掌楸树花的螺原体菌株SE - 3密切相关。
