Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
J Invertebr Pathol. 2012 Jan;109(1):172-4. doi: 10.1016/j.jip.2011.11.006. Epub 2011 Nov 22.
Spiroplasma apis and Spiroplasma melliferum are known as honey bee pathogens and are detected by unspecific methodologies like culturing or dark field microscopy. We developed a multiplex PCR being able to differentiate between both species and detect the genus Spiroplasma. This PCR can directly be used on culture samples or on DNA extracted bees. By PCR on cultured samples we were able to identify S. apis in Bombus pratorum and S. melliferum in Bombus pascuorum.
蜜蜂螺旋体（Spiroplasma apis）和蜜蜂光泽螺旋体（Spiroplasma melliferum）是已知的蜜蜂病原体，可通过培养或暗视野显微镜等非特异性方法检测到。我们开发了一种多重 PCR 方法，能够区分这两种物种，并检测出螺旋体属。该 PCR 可直接用于培养样本或提取的蜜蜂 DNA。通过对培养样本的 PCR 分析，我们在熊蜂属的熊蜂巴氏亚种（Bombus pratorum）中鉴定出了 S.apis，在熊蜂属的熊蜂坎伯尔亚种（Bombus pascuorum）中鉴定出了 S. melliferum。
