Silinskas K C, Kateley S A, Tower J E, Maher V M, McCormick J J
Cancer Res. 1981 May;41(5):1620-7.
We have demonstrated a dose-dependent increase in the frequency of diploid human cells capable of anchorage-independent (AI) growth after treatment with the carcinogen propane sultone, followed by exponential growth to allow full expression of this phenotype (8 to 13 population doublings). Exposure to these same concentrations of propane sultone also resulted in a dose-dependent increase in the frequency of 6-thioguanine-resistant cells in the population. Procedures such as synchronization of cells and treatment just after the onset of DNA synthesis or the use of special selective medium were not essential for this induction. A very low frequency of cells with the AI phenotype was found in the control population (background). Cells which exhibited the AI phenotype spontaneously or after carcinogen treatment retained the characteristic over as many generations as tested (greater than 13). The data suggest that AI growth is the result of a mutational event.
我们已经证明，在用致癌物丙烷磺内酯处理后，能够进行不依赖贴壁（AI）生长的二倍体人类细胞频率呈剂量依赖性增加，随后进行指数生长以充分表达这种表型（8至13个群体倍增）。暴露于相同浓度的丙烷磺内酯也导致群体中6-硫代鸟嘌呤抗性细胞的频率呈剂量依赖性增加。细胞同步化以及在DNA合成开始后立即进行处理或使用特殊选择培养基等程序对于这种诱导并非必不可少。在对照群体（背景）中发现具有AI表型的细胞频率非常低。自发或经致癌物处理后表现出AI表型的细胞在所测试的许多代中（超过13代）都保留了该特征。数据表明AI生长是突变事件的结果。
