Van Veelen C W, Rijksen G, Vlug A M, Staal G E
Clin Chim Acta. 1981 Feb 19;110(1):113-20. doi: 10.1016/0009-8981(81)90307-7.
A method is described for determination of the percentage of K-subunits in the K-M set of pyruvate kinase. The method is based on the alanine inhibition of the K-subunits. A linear correlation (r = 0.99) is found between the alanine inhibition and the percentage of K-subunits as calculated from the electropherograms, assuming (a) a subunit distribution as indicated by the suffix in K4, K3M, K2M2, etc., and (b) an equal contribution of K- and M-subunits to the intensity of the stain.
描述了一种测定丙酮酸激酶K-M同工酶中K亚基百分比的方法。该方法基于丙氨酸对K亚基的抑制作用。假定（a）K4、K3M、K2M2等后缀所表示的亚基分布，以及（b）K亚基和M亚基对染色强度的贡献相等，则丙氨酸抑制作用与根据电泳图谱计算出的K亚基百分比之间存在线性相关性（r = 0.99）。
