ElRehewy M, Kong Y M, Giraldo A A, Rose N R
Eur J Immunol. 1981 Feb;11(2):146-51. doi: 10.1002/eji.1830110216.
Mouse thyroglobulin (MTg) or thyroid extract (TE) was given repeatedly to good responder C3H/Anf (H-2k) and poor responder BALB/c (H-2d) mice in the absence of adjuvant. Anti-MTg antibodies reached high levels in good responder mice given high doses of thyroid antigen. To eliminate stimulation by alloantigenic determinants and reduce the chance of denaturation. TE from syngeneic mice was prepared freshly each week and injected into good and poor responder strains. Again, significant antibody titers were observed in good responder mice. The antibody was specific for MTg since (a) it was not inhibited by extracts of other organs and (b) it reacted strongly with the closely related rat Tg and weakly with Tg from other species. Histology revealed mononuclear cell infiltration of the thyroid of good responder, but not of poor responder mice, regardless of the strain used to provide the thyroid antigen. The data demonstrate the presence of both T and B cell populations reactive with this self antigen and their stimulation by repeated high doses of antigen, without the aid of adjuvant, to override the regulatory controls that normally prevent autoimmune responses.
在无佐剂的情况下，将小鼠甲状腺球蛋白（MTg）或甲状腺提取物（TE）反复给予反应良好的C3H/Anf（H-2k）小鼠和反应不佳的BALB/c（H-2d）小鼠。给予高剂量甲状腺抗原时，反应良好的小鼠体内抗MTg抗体达到高水平。为消除同种异体抗原决定簇的刺激并降低变性的可能性，每周新鲜制备同基因小鼠的TE，并注射到反应良好和反应不佳的品系中。同样，在反应良好的小鼠中观察到显著的抗体滴度。该抗体对MTg具有特异性，因为（a）它不受其他器官提取物的抑制，（b）它与密切相关的大鼠Tg强烈反应，与其他物种的Tg反应较弱。组织学显示，无论用于提供甲状腺抗原的品系如何，反应良好的小鼠甲状腺有单核细胞浸润，而反应不佳的小鼠则没有。数据表明存在与这种自身抗原反应的T细胞和B细胞群体，以及它们在无佐剂情况下通过反复高剂量抗原刺激，以克服通常阻止自身免疫反应的调节控制。
