Bonte W, Jursch R, Straube J
Forensic Sci Int. 1981 Jan-Feb;17(1):45-9. doi: 10.1016/0379-0738(81)90188-2.
A method for the identification and quantitation of hemoglobin derivatives is described. Samples diluted 1 in 100 were applied to precoated polyacrylamide-gel plates containing an Ampholine gradient of pH 3.5 - 9.5 and focused for 1.5 h at a constant 40 W. All hemoglobins under investigation wee characterized by specific patterns which allow them to be differentiated. Electrofocusing is recommended for screening tests, but quantitative evaluation using densitometry is possible as well.
本文描述了一种鉴定和定量血红蛋白衍生物的方法。将稀释为1:100的样品应用于预先包被的含有pH 3.5 - 9.5两性电解质梯度的聚丙烯酰胺凝胶板上，并在40 W恒定功率下聚焦1.5小时。所有被研究的血红蛋白都具有特定的模式特征，这使得它们能够被区分开来。推荐使用电聚焦进行筛选测试，但也可以使用光密度测定法进行定量评估。
