通过等电聚焦鉴定物种特异性血红蛋白。
Identification of species specific hemoglobin by isoelectric focusing.
作者信息
Oshima M, Inoue T, Hara M
出版信息
Forensic Sci Int. 1982 Nov-Dec;20(3):277-86. doi: 10.1016/0379-0738(82)90129-3.
A method for the discrimination of human hemoglobin from animal sources is described. Hemoglobins from 34 species with and without treatment by p-chloromercuribenzoate (PCMB) were examined by thin-layer isoelectric focusing (IEF) in polyacrylamide gel (PAG) plates containing an Ampholine gradient of pH 3.5 - 9.5. PCMB treatment was effective in distinguishing human and animal hemoglobins with their characteristic IEF patterns. Values for the isoelectric points of the major and minor hemoglobins of each species are also described.
本文描述了一种区分人源血红蛋白与动物源血红蛋白的方法。采用含有pH 3.5 - 9.5两性电解质梯度的聚丙烯酰胺凝胶（PAG）板进行薄层等电聚焦（IEF），对34种经过和未经过对氯汞苯甲酸（PCMB）处理的血红蛋白进行了检测。PCMB处理能有效地通过其特征性IEF图谱区分人和动物的血红蛋白。文中还描述了每个物种主要和次要血红蛋白的等电点值。
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