Ogg J E, Timme T L, Alemohammad M M
Infect Immun. 1981 Feb;31(2):737-41. doi: 10.1128/iai.31.2.737-741.1981.
Evidence was obtained for general transduction in Vibrio cholerae. Transduction of three amino acid markers and three antibiotic resistance characters was demonstrated using strains of biotype eltor and biotype cholerae. Some of the genetic characters were transduced from a biotype eltor donor (and its mutant derivatives) to biotype cholerae and eltor recipients. For the genetic traits examined, the frequencies of transduction ranged between 10(-5) and 10(-8). Maximal frequencies were obtained with transducing phage lysates that were irradiated with ultraviolet light. The development of a system of general transduction will now aid in fine structure analysis and detailed mapping of the chromosome of V. cholerae.
已获得霍乱弧菌中普遍转导的证据。使用埃尔托生物型和霍乱生物型菌株证明了三种氨基酸标记和三种抗生素抗性特征的转导。一些遗传特征从埃尔托生物型供体（及其突变衍生物）转导至霍乱生物型和埃尔托生物型受体。对于所检测的遗传性状，转导频率在10^(-5)至10^(-8)之间。用紫外线照射的转导噬菌体裂解物可获得最高频率。普遍转导系统的建立现在将有助于霍乱弧菌染色体的精细结构分析和详细图谱绘制。
