Ivanovich E, Antov G, Kazakova B
Int Arch Occup Environ Health. 1981;48(1):41-7. doi: 10.1007/BF00405930.
Liver changes after separate and combined action of vibration (whole body, 100 Hz, 0.1 mm amplitude) and other factors: noise (white noise 105 dB/A), heat (35 degrees C, humidity 45--55% and air velocity 0.2--0.3 ms-1) and lead (lead acetate, 20 mg/kg) were studied in white rats. The exposure lasted for 2 h daily during 10 days (lead was daily applied per os in a water solution). After the treatment in liver homogenates the activity of SucDH, LDH, and ATP-ase, as well as the quantity of soluble proteins (SP) and -SH gr were determined. In fresh frozen liver slices the activity of SucDH, LDH, and ATP-ase were investigated. Liver samples were studied by light and electro-microscopy. The results show that vibration alone did not produce marked changes, but when the other factors acted simultaneously, more expressed alterations in the liver were found. The most pronounced changes were obtained after vibration and lead effect. The histological, histochemical, and electron-microscopic findings support the biochemical data about certain disturbances in the energy supply and utilization in the liver tissue.
研究了振动（全身振动，100赫兹，振幅0.1毫米）与其他因素：噪声（白噪声105分贝/A）、热（35摄氏度，湿度45 - 55%，风速0.2 - 0.3米/秒）和铅（醋酸铅，20毫克/千克）单独及联合作用对大白鼠肝脏的影响。暴露持续10天，每天2小时（铅每天经口以水溶液形式给予）。处理后，测定肝脏匀浆中琥珀酸脱氢酶（SucDH）、乳酸脱氢酶（LDH）和ATP酶的活性，以及可溶性蛋白（SP）和巯基（-SH）的含量。在新鲜冷冻的肝脏切片中研究了SucDH、LDH和ATP酶的活性。通过光学显微镜和电子显微镜对肝脏样本进行研究。结果表明，单独的振动不会产生明显变化，但当其他因素同时作用时，肝脏中会出现更明显的改变。振动和铅作用后变化最为显著。组织学、组织化学和电子显微镜检查结果支持了关于肝脏组织能量供应和利用方面某些紊乱的生化数据。
