Goosey J D, Zigler J S, Matheson I B, Kinoshita J H
Invest Ophthalmol Vis Sci. 1981 May;20(5):679-83.
We have recently demonstrated that singlet oxygen-mediated photooxidation can cause an increase in blue fluorescence and covalent cross-linking in bovine lens crystallins in vitro. Because these changes closely parallel modifications known to occur in human crystallins during aging and cataractogenesis, it was suggested that singlet oxygen may play an important role in these processes in vivo. to confirm these results, obtained with photosensitizers free in solution, we now report results from experiments with a polymer-bound photosensitizer and with photophysically generated singlet oxygen. Singlet oxygen photodynamically generated by polymer-immobilized rose bengal produced the following modifications to human lens crystallins in vitro: covalent cross-linking, increased blue fluorescence, yellow pigmentation, and formation of heavy-molecular-weight aggregates. Singlet oxygen generated photophysically was shown to cause an increase in blue fluorescence and covalent cross-linking in human crystallins. These findings substantiate the hypothesis that singlet oxygen may play an important role in the changes seen in human lens proteins during aging and cataractogenesis.
我们最近证明，单线态氧介导的光氧化可在体外使牛晶状体晶状体蛋白的蓝色荧光增强并发生共价交联。由于这些变化与已知在人类晶状体蛋白老化和白内障形成过程中发生的修饰密切相似，因此有人提出单线态氧可能在体内这些过程中起重要作用。为了证实这些用游离于溶液中的光敏剂获得的结果，我们现在报告使用聚合物结合光敏剂和光物理产生的单线态氧进行实验的结果。由聚合物固定的孟加拉玫瑰红光动力产生的单线态氧在体外对人晶状体晶状体蛋白产生了以下修饰：共价交联、蓝色荧光增强、黄色色素沉着以及重分子量聚集体的形成。光物理产生的单线态氧被证明可导致人晶状体蛋白的蓝色荧光增强和共价交联。这些发现证实了单线态氧可能在人类晶状体蛋白老化和白内障形成过程中所见变化中起重要作用的假设。
