Rundquist I
Histochemistry. 1981;70(2):151-9. doi: 10.1007/BF00493207.
An automated microscope fluorometer based on a modified Leitz MPV I system is described. Automation is mainly attained by the use of a cheap, commercially available personal computer system programmed in extended BASIC supplemented by a few assembly-level subroutines. The automation has resulted in simplified measuring procedures and increased measuring speed. The statistical precision is enhanced because greater cell populations can be analysed within reasonable time. Programs for several biological applications are described. Routines for cell population analysis, including storage of data on disk files, has proved especially useful. Programs for recording of corrected fluorescence emission spectra and of time-dependent fluorescence variables have also been developed. A high flexibility is achieved, as the BASIC programs can readily be modified for specific purposes. Some data on the sensitivity and reproducibility of the instrument alone, and in combination with a staining method, are also presented.
本文描述了一种基于改进型徕卡MPV I系统的自动显微镜荧光计。自动化主要通过使用一台价格低廉、可商购的个人计算机系统来实现，该系统用扩展BASIC编程，并辅以一些汇编级子程序。自动化带来了测量程序的简化和测量速度的提高。由于能够在合理时间内分析更多细胞群体，统计精度得到了提高。文中描述了几个生物学应用程序。细胞群体分析程序，包括将数据存储到磁盘文件中，已证明特别有用。还开发了用于记录校正后的荧光发射光谱和随时间变化的荧光变量的程序。由于BASIC程序可以很容易地针对特定目的进行修改，因此实现了高度的灵活性。文中还给出了该仪器单独使用以及与一种染色方法结合使用时的一些灵敏度和重现性数据。
