Noguchi C T, Torchia D A, Schechter A N
J Biol Chem. 1981 May 10;256(9):4168-71.
We report 13C/1H magnetic double resonance measurements of polymer in sickle trait erythrocytes and direct measurements of solubility on lysates of these cells as a function of oxygen saturation. Polymerized hemoglobin is detected by these two methods only at oxygen saturation values below 60% and increases to 0.4 of the total hemoglobin at complete deoxygenation. These results confirm the validity of the NMR approach in determining values of polymer fraction. We have also found that the thermodynamic theory, that successfully describes gelation in sickle erythrocytes, does not predict the experimental results for sickle trait erythrocytes and must be refined.
我们报告了对镰状性状红细胞中聚合物的¹³C/¹H 磁双共振测量，以及作为氧饱和度函数对这些细胞裂解物溶解度的直接测量。仅在氧饱和度值低于60%时，通过这两种方法才能检测到聚合血红蛋白，在完全脱氧时其增加至总血红蛋白的0.4。这些结果证实了核磁共振方法在确定聚合物分数值方面的有效性。我们还发现，成功描述镰状红细胞凝胶化的热力学理论并不能预测镰状性状红细胞的实验结果，必须加以完善。
