Ponder C
J Assoc Off Anal Chem. 1978 Sep;61(5):1089-91.
A fluorometric method is described for determining indole in shrimp. The indole is extracted with n-hexane, partitioned into a methanol-saturated sodium chloride solution (9+1), and determined fluorometrically. The detection limit of the method is 0.04 microgram indole/g shrimp. Quantitative analytical data are presented for indole in samples of shrimp determined by the fluorometric method and the AOAC official colorimetric and gas-liquid chromatographic procedures. The correlation coefficient between the data of the fluorometric and colorimetric methods was 0.96. Indole recovered from 25 g samples of fresh shrimp spiked with 6 and 12 microgram indole/25 g ranged from 97 to 106%. The fluorescence response is linear in the range of 1 to 25 microgram indole/100 ml methanol solution and no significant change is noted in solutions kept in the dark 18 hr.
描述了一种用于测定虾中吲哚的荧光法。吲哚用正己烷萃取，分配到甲醇饱和氯化钠溶液（9 + 1）中，然后进行荧光测定。该方法的检测限为0.04微克吲哚/克虾。给出了通过荧光法以及美国官方分析化学师协会（AOAC）的比色法和气液色谱法测定的虾样品中吲哚的定量分析数据。荧光法和比色法数据之间的相关系数为0.96。从添加了6和12微克吲哚/25克的25克鲜虾样品中回收的吲哚范围为97%至106%。荧光响应在1至25微克吲哚/100毫升甲醇溶液范围内呈线性，并且在黑暗中保存18小时的溶液中未观察到显著变化。
