胆汁色素的高效液相色谱法：应用于血清中未结合胆红素的测定
High-performance liquid chromatography of bile pigments: application to the determination of unconjugated bilirubin in serum.
作者信息
Lim C K, Bull R V, Rideout J M
出版信息
J Chromatogr. 1981 Jan 16;204:219-23. doi: 10.1016/s0021-9673(00)81662-3.
PMID:7217254
Abstract
A rapid, sensitive and specific method for the determination of unconjugated bilirubin in serum is described. Reversed-phase chromatography on short-chain alkylsilica (C2) or octadecylsilica (C18) with acetonitrile--dimethylsulphoxide--water as eluent is used for the separation of bilirubin. The method is applied to the analysis of bilirubin in patients with disorders associated with jaundice.
摘要
本文描述了一种用于测定血清中未结合胆红素的快速、灵敏且特异的方法。采用短链烷基硅胶（C2）或十八烷基硅胶（C18）进行反相色谱分离，以乙腈 - 二甲基亚砜 - 水作为洗脱剂来分离胆红素。该方法应用于患有黄疸相关病症患者的胆红素分析。
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