Nimura N, Ishida K, Kinoshita T
J Chromatogr. 1980 Dec 12;221(2):249-55. doi: 10.1016/s0378-4347(00)84309-1.
A novel method is described in which catecholamines are converted into fluorescent products by heating in alkaline borate buffer. The method was applied to the determination of norepinephrine and epinephrine after separation by high-performance liquid chromatography using a pellicular, strong cation exchanger. The new system is simpler than the system based on the trihydroxyindole reaction. It is suitable for the measurement of catecholamines in the range of 0.25-20 ng. The assay of catecholamines in human urine is also described.
本文描述了一种新方法，即在碱性硼酸盐缓冲液中加热，将儿茶酚胺转化为荧光产物。该方法应用于使用薄壳型强阳离子交换剂通过高效液相色谱分离后去甲肾上腺素和肾上腺素的测定。新系统比基于三羟基吲哚反应的系统更简单。它适用于0.25 - 20 ng范围内儿茶酚胺的测量。本文还描述了人尿中儿茶酚胺的测定方法。
