DeAngelis R L, Robinson M M, Brown A R, Johnson T E, Welch R M
J Chromatogr. 1980 Dec 12;221(2):353-60. doi: 10.1016/s0378-4347(00)84321-2.
A quantitative thin-layer chromatography (TLC) procedure is described for the analysis of cinromide (3-bromo-N-ethylcinnamamide) and its two major metabolites, 3-bromocinnamamide and 3-bromocinnamic acid in plasma of the dog. These compounds were recovered from acidified plasma by extraction into benzene with a recovery of 95 +/- 5%. All three compounds were quantitated directly on a TLC plate by ultraviolet absorbance densitometry at 270 nm. The linear dynamic range for the quantitation of the compounds on a TLC plate ranged between 10 and 1000 ng. The complete procedure is useful in the working range of 50 ng/ml to 100 microgram/ml of plasma with a coefficient of variability of about 10%. Specificity of the method for parent drug and each of its plasma metabolites was confirmed by high-performance liquid chromatography. The method was used to determine the pharmacokinetics of cinromide and its two major plasma metabolites in dogs following a single oral dose of the drug.
描述了一种定量薄层色谱（TLC）方法，用于分析犬血浆中的西诺米特（3-溴-N-乙基肉桂酰胺）及其两种主要代谢物3-溴肉桂酰胺和3-溴肉桂酸。通过用苯萃取从酸化血浆中回收这些化合物，回收率为95±5%。所有三种化合物都通过在270nm处的紫外吸收光密度法直接在TLC板上进行定量。TLC板上化合物定量的线性动态范围在10至1000ng之间。整个方法在血浆浓度为50ng/ml至100μg/ml的工作范围内有用，变异系数约为10%。通过高效液相色谱法确认了该方法对母体药物及其每种血浆代谢物的特异性。该方法用于测定犬单次口服该药物后西诺米特及其两种主要血浆代谢物的药代动力学。
