Honikel K O, Schmidt U, Woltersdorf W, Leistner L
J Assoc Off Anal Chem. 1978 Sep;61(5):1222-7.
A semiquantitative microbiological screening test for antibiotics, a sensitive and quantitative microbiological assay, and a fluorometric method specific for tetracyclines are described. Using these procedures, tetracycline residues in animals derived from feed can be detected in tissues like organs, muscles, and bones. Meat contaminated with chlortetracycline (CTC) and oxytetracycline (OTC) and stored at +8 degrees C and -22 degrees C showed very little decrease in antibiotic concentration; however, heating above 65 degrees C reduced the tetracycline content in meat. Temperatures above 130 degrees C were necessary to destroy CTC in bones, CTC in bones was insoluble above pH 4. Manufacturing products with contaminated meat reduced the tetracycline content only if heating was involved.
本文描述了一种用于抗生素的半定量微生物筛选试验、一种灵敏的定量微生物测定法以及一种针对四环素的荧光测定法。使用这些方法，可以在动物的器官、肌肉和骨骼等组织中检测到源自饲料的四环素残留。被金霉素（CTC）和土霉素（OTC）污染并储存在8摄氏度和零下22摄氏度的肉类，其抗生素浓度几乎没有下降；然而，加热到65摄氏度以上会降低肉中的四环素含量。要破坏骨骼中的CTC，温度必须高于130摄氏度，骨骼中的CTC在pH值高于4时不溶解。只有在涉及加热的情况下，用受污染的肉制造产品才会降低四环素含量。
