Korimová L, Máté D, Turek P, Sokol J
University of Veterinary Medicine, Kosice, Slovak Republic.
Vet Med (Praha). 1997 May;42(5):129-31.
HPLC method with solid phase extraction, and microbiological agar diffusion tests for determination of oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) in bones, mechanically separated beef and bones were developed. Both mechanically separated bones and bovine flat bones showed the highest yield of tetracyclines by HPLC method. Among tetracyclines, the highest yield was determined in chlortetracycline. The strains of Bacillus subtilis (PH 6.0) and B. stearothermophilus showed the highest sensitivity to the presence of tetracyclines.
建立了用于测定骨骼、机械分离牛肉和骨骼中土霉素（OTC）、四环素（TC）和金霉素（CTC）的固相萃取高效液相色谱法以及微生物琼脂扩散试验。机械分离的骨骼和牛扁骨通过高效液相色谱法显示出四环素的最高回收率。在四环素中，金霉素的回收率最高。枯草芽孢杆菌（PH 6.0）和嗜热脂肪芽孢杆菌菌株对四环素的存在表现出最高的敏感性。
