Yamanaka M, Nomura T, Tokioka J, Kametaka M
J Nutr Sci Vitaminol (Tokyo). 1980;26(5):435-47. doi: 10.3177/jnsv.26.435.
An amino acid mixture diet(AA) simulated with purified whole-egg protein and a purified whole-egg protein diet(WE) were given to ICR strain male germ-free(GF) and conventional(CV) mice for three weeks from five to eight weeks of age. All mice were killed at eight weeks of age and the gastrointestinal tracts were removed. The lengths of the small intestine were measured and six parts, i.e., the stomach; upper, middle and lower parts of the small intestine; cecum; and colon and rectum were separated, and each part (with contents) was weighed immediately. The contents were removed from each part by washing with distilled water. For the gut without contents, only total nitrogen(TN) was estimated and for the gut contents, TN, protein nitrogen(PN) and water-insoluble nitrogen(WIN) were estimated. The fresh weight of cecum with contents per 100 g of body weight of GF mice fed on AA diet, 2.07 +/- 0.11 g (mean +/- SE), was lighter than that of GF mice fed on WE diet, which was 4.51 +/- 0.28 g. The weight and length of the small intestine of AA diet groups were smaller than those of WE diet group. TN and PN(mg) in whole gut contents per 100 g of body weight in the WE diet group were higher than those in the AA diet group and high TN and PN were observed in GF mice. The PN per TN and WIN per PN of gut contents were affected more by the presence of the flora than by the difference in diets, and CV mice showed high values. The TN of the intestinal tract excluding contents showed no differences in GF and CV mice fed on either diet.
从5至8周龄起，将用纯化全蛋蛋白模拟的氨基酸混合饮食（AA）和纯化全蛋蛋白饮食（WE）分别给予ICR品系雄性无菌（GF）小鼠和常规（CV）小鼠，持续三周。所有小鼠在8周龄时处死，取出胃肠道。测量小肠长度，分离出六个部分，即胃；小肠的上、中、下部分；盲肠；以及结肠和直肠，每个部分（连同内容物）立即称重。通过用蒸馏水冲洗从每个部分去除内容物。对于没有内容物的肠道，仅估算总氮（TN），对于肠道内容物，估算TN、蛋白氮（PN）和水不溶性氮（WIN）。喂食AA饮食的GF小鼠每100克体重含内容物的盲肠鲜重为2.07±0.11克（平均值±标准误），低于喂食WE饮食的GF小鼠，后者为4.51±0.28克。AA饮食组小肠的重量和长度小于WE饮食组。WE饮食组每100克体重全肠道内容物中的TN和PN（毫克）高于AA饮食组，并且在GF小鼠中观察到高TN和PN。肠道内容物的PN/TN和WIN/PN受菌群存在的影响大于饮食差异的影响，CV小鼠显示出较高的值。不含内容物的肠道的TN在喂食任何一种饮食的GF和CV小鼠中均无差异。
